Immunochemical studies of organ and tumor lipids. XIX. Cytolytic action of antibodies directed against cytolipin R.

Immunochemical studies of organ and tumor lipids . XIX . Cytolytic

Rabbit antisera to rat lymphosarcoma contain antibodies that are cytolytic for rat erythrocytes in the presence of complement. The reaction can be inhibited completely by pure cytolipin R showing that (u) immune hemolysis can be mediated through lipid determinants in the membrane, and ( b ) that cytolipin R determinants are present in the intact erythrocyte membrane and exposed on the surface. ...

Immunochemical studies of organ and tumor lipides. X. The presence of a novel lipide hapten, cytolipin G, in tumors and tissues of the gastrointestinal tract.

Lipidcs extracted from human carcinomas of the stomach, small intestine, and colon wcrc fract ionated on silicic acid columns and the fractions tested for serological act ivi ty with two antisera: one containing anticytolipin II an(] the other devoid of this ant ibody but reactive with crude lipidcs of gastrointestinal tissues, both normal and neoplastic. Two different haptens could be demonstr...

تولید و تخلیص آنتی بادی پلی کلونال بر علیه هورمون ملاتونین

Nowadays immunochemical techniques have played a very important and valuable role in quantitative and qualitative assays of liquid compounds of the body. Producing antibody against immunogenes is the first step to make immunochemical kits. In this study production and purification of polyclonal antibody against melatonin has been considered. This hormone which has several important functions in...

Characterization of IgY antibodies, developed in hens, directed against camel immunoglobulins

Chicken egg yolk antibodies (IgY) against camel immunoglobulins were generated and their specificity was shown by double immunodiffusion, immunoelectrophoresis, and Western blotting. Anti camel antibodies specifically react to camel antibodies in immunodiffusion and immunoelectrophoresis were demonstrated. Western blotting revealed that IgY antibodies recognize the heavy chain in a number of ma...

Immunochemical studies of organ and tumor lipids: VIII. Comparison of human tumor and ox spleen cytosides.